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Gene integration into bovine sperm genome and its expression in transgenic offspring
Author(s) -
Shemesh M.,
Gurevich M.,
HarelMarkowitz E.,
Benvenisti L.,
Shore L.S.,
Stram Y.
Publication year - 2000
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(200006)56:2+<306::aid-mrd21>3.0.co;2-3
Subject(s) - biology , sperm , transgene , restriction enzyme , southern blot , offspring , microbiology and biotechnology , gene , embryo , exogenous dna , andrology , genetics , genomic dna , genetically modified mouse , medicine , pregnancy
Transgenic bovine sperm were produced by restriction enzyme mediated insertion (REMI). REMI utilizes lipofection of linearized pEGFP and the corresponding restriction enzyme for integration into the sperm genomic DNA. The transgenic sperm were used in IVF to produce morula expressing GFP. When transgenic sperm were used for AI in two cows, the resultant calves expressed the exogenous DNA in their lymphocytes as determined by (a) PCR and RT‐PCR; (b) specific emission of green fluorescence by GFP; and (c) Southern blot analysis. Data demonstrate that REMI is an efficient method for the production of transgenic sperm and corresponding offspring by AI or embryos by IVF. Mol. Reprod. Dev. 56:306–308, 2000. © 2000 Wiley‐Liss, Inc.

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