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Two distinct c‐ ski cDNAs of fish, tilapia ( Oreochromis aurea )
Author(s) -
Huang ChiuJu,
Lin JerYoung,
Tsai HuaiJen
Publication year - 1999
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199911)54:3<223::aid-mrd3>3.0.co;2-g
Subject(s) - biology , tilapia , oocyte , xenopus , ovary , complementary dna , microbiology and biotechnology , oreochromis , andrology , gene , fish <actinopterygii> , endocrinology , embryo , genetics , fishery , medicine
Two classes of tilapia c‐ ski cDNA (accession nos. AJ012011, AJ012012), designated as t ski 1 and t ski 2, respectively encoded a 687 and a 714 AA protein and shared a 57% AA identity. Comparison with the Ski proteins of chickens, humans and Xenopus , tilapia TSki polypeptides shared a 60, 57, and 57% (TSki1) and 67, 63, and 61% (TSki2) AA identity, respectively. The most and the least abundant c‐ ski mRNAs are located in the brain and the skeletal muscle, respectively. Both t ski 1 and t ski 2 were widely expressed in the adult tissues examined, but t ski 2 transcripts were at higher levels except in the ovary and oocytes: t ski 1 transcripts were predominant in the ovary, whereas t ski 2 transcripts were predominant in the testes. In the oocytes, the t ski 1 mRNA was a maternally‐inherited stockpile that subsequently was degraded, so that the expression ratio of t ski 1 to t ski 2 transcripts declined gradually as the fish developed from oocyte to 4‐cm fry. Mol. Reprod. Dev. 54:223– 231. © 1999 Wiley‐Liss, Inc.