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Premium Analysis of control elements for position‐independent expression of human α‐lactalbumin YAC
Fujiwara Yoshihiro,
Takahashi RiIchi,
Miwa Masami,
Kameda Midori,
Kodaira Kunihiko,
Hirabayashi Masumi,
Suzuki Takashige,
Ueda Masatsugu
Publication year1999
Publication title
molecular reproduction and development
Resource typeJournals
PublisherJohn Wiley & Sons
Abstract A major problem in the production of transgenic animal bioreactors using microinjections is the low production rate of high‐expressing transgenic animals due to the position effect. We previously reported that transgenic rats carrying the 210 kb yeast artificial chromosome (YAC) including the human α‐lactalbumin gene express the transgene in a position‐independent manner. The 210 kb YAC was thought to have all the elements necessary for position‐independent expression. In this paper, we constructed fragmented YAC clones and a cosmid clone, and produced transgenic rats to analyze these elements. Transgenic rats with both the 50 kb upstream and downstream regions of the α‐lactalbumin gene had position‐independent expression. Transgenic rats with the 20 kb upstream and downstream regions, however, had position‐dependent expression. Therefore, all the elements necessary for position‐independent expression are thought to be located in the 50 kb upstream to 50 kb downstream region of the α‐lactalbumin gene. Furthermore, we replaced the human α‐lactalbumin promoter with the bovine αS1‐casein promoter in the 210 kb YAC and produced transgenic rats. Position‐dependent expression was observed. The elements required for position‐independent expression of the bovine αS1‐casein gene are different from those required for the human α‐lactalbumin gene, despite the fact that the two genes have the same tissue and developmental specificity. Mol. Reprod. Dev. 54:17–23, 1999. © 1999 Wiley‐Liss, Inc.
Subject(s)biochemistry , biology , chromosome , gene , gene expression , gene mapping , genetics , lactalbumin , microbiology and biotechnology , position effect , promoter , transgene , yeast artificial chromosome
SCImago Journal Rank0.745

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