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Simultaneous detection of X‐ and Y‐bearing bull spermatozoa by double colour fluorescence in situ hybridization
Author(s) -
Hassanane Mohamed,
Kovacs Andras,
Laurent Pascal,
Lindblad Kerstin,
Gustavsson Ingemar
Publication year - 1999
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199908)53:4<407::aid-mrd6>3.0.co;2-o
Subject(s) - biology , fluorescence in situ hybridization , sperm , ploidy , in situ hybridization , y chromosome , in situ , x chromosome , cosmid , chromosome , fluorescence , microbiology and biotechnology , genetics , dna , gene , optics , gene expression , physics , meteorology
Double colour fluorescence in situ hybridization with sex chromosome probes was applied on sperm cells of five Swedish Holstein‐Friesian bulls. It was demonstrated that cosmids with strong fluorescence signals and scraped chromosomes can successfully be used as markers in this type of study. X and Y segregated as expected according to a 1:1 ratio, and there were no interindividual variations. There was a tendency for there to be more Y‐ than X‐bearing spermatozoa, but this bias was assumed to be due to the markers used. Disomic spermatozoa occurred with a frequency of more than 0.1 % (0.067% XX, 0.029% YY, and 0.029% XY), which is considerably lower than the frequency in humans. Diploid sperm cells occurred with a frequency of 0.05 %. Mol. Reprod. Dev. 53:407–412, 1999. © 1999 Wiley‐Liss, Inc.