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Temperature‐dependent osmotic behavior of germinal vesicle and metaphase II stage bovine oocytes in the presence of Me 2 SO in relationship to cryobiology
Author(s) -
Agca Y.,
Liu J.,
Critser E.S.,
McGRATH J.J.,
Critser J.K.
Publication year - 1999
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199905)53:1<59::aid-mrd7>3.0.co;2-e
Subject(s) - germinal vesicle , arrhenius plot , biology , arrhenius equation , cryobiology , vitellogenin , cryopreservation , oocyte , oogenesis , activation energy , analytical chemistry (journal) , andrology , microbiology and biotechnology , chromatography , biochemistry , chemistry , embryo , genetics , organic chemistry , gene , medicine
Plasma membrane permeability coefficients and their activation energies (E a ) for water (L p ) and dimethyl sulfoxide (P Me 2 SO ) as well as the reflection coefficient (σ) were determined for germinal vesicle (GV) and metaphase II (MII) bovine oocytes. A micropipette perfusion technique was used with a temperature controlled circulation chamber, which was adapted to a micromanipulator. Experiments were performed at five different temperatures (30, 20, 10, 4 and –3°C). The Kedem and Katchalsky model was assumed and L p , (P Me 2 SO ) and σ were estimated. Estimated permeability values from the experimental temperatures were then applied to Arrhenius plots ln(L p ) or ln(P Me 2 SO ) vs 1/Temperature (K) to estimate the activation energies (E a ) for L p Me 2 SOand P Me 2 SO . The estimated E a for L p Me 2 SOfor GV and MII oocytes were 23.84 Kcal/mol and 8.46 Kcal/mol, respectively. The E a for P Me 2 SOwere 21.0 Kcal/mol and 23.20 Kcal/mol, respectively. The correlation (r 2 ) for these linear regression plots for GV oocytes were 0.83 and 0.95 for L p Me 2 SOand P Me 2 SO , respectively. For MII oocytes, r 2 values were 0.95 and 0.99 for L p Me 2 SOand P Me 2 SO , respectively. There was a possible discontinuity detected in the Arrhenius plot for L p Me 2 SOfor GV oocytes. A significant decrease of the reflection coefficient was observed at 10°C compared to other experimental temperatures. These data provide a fundamental basis that should be taken into account for low temperature preservation of bovine oocytes in the presence of Me 2 SO. Mol. Reprod. Dev. 53:59–67, 1999. © 1999 Wiley‐Liss, Inc.