Premium
Protein phosphorylation is essential for formation of male pronucleus in bovine oocytes
Author(s) -
Chian R.C.,
Tan S.L.,
Sirard M.A.
Publication year - 1999
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199901)52:1<43::aid-mrd6>3.0.co;2-c
Subject(s) - pronucleus , biology , male pronucleus , andrology , phosphorylation , insemination , human fertilization , okadaic acid , zygote , sperm , microbiology and biotechnology , genetics , embryo , embryogenesis , phosphatase , medicine
This study examined the association between the morphological and protein phosphorylation events following sperm penetration of in vitro matured and in vitro fertilized bovine oocytes. Oocytes were labeled with [ 32 P]‐orthophosphate at 3 hr intervals from 3 to 18 hr of following insemination. The phosphorylation of protein complexes of 23 kDa and 18 kDa specifically increased with the formation of male and female pronuclei. In addition, oocytes were treated with 6‐Dimethylaminopurine (6‐DMAP) or Okadaic acid (OA) at 0, 3, 6, and 9 hr respectively following insemination. Although the formation of female pronucleus was not affected by 6‐DMAP, the male pronuclear formation was completely inhibited by the presence of 6‐DMAP at 0 and 3 hr of post insemination. The formation of both pronuclei was inhibited by the presence of OA at any time following insemination. These results suggest that the male pronuclear formation is associated with protein phosphorylation and that the formation of the male and the female pronuclei may involve different factors in bovine zygotes since they respond differently to the kinase modulations. Mol. Reprod. Dev. 52:43–49, 1999. © 1999 Wiley‐Liss, Inc.