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DNA ploidy abnormalities in rabbit preimplantation embryos are not increased by conditions associated with in vitro culture
Author(s) -
Schumacher Armin,
Kesdogan Jadigar,
Fischer Bernd
Publication year - 1998
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199805)50:1<30::aid-mrd4>3.0.co;2-y
Subject(s) - biology , ploidy , embryo , in vitro , andrology , dna , rabbit (cipher) , genetics , microbiology and biotechnology , gene , medicine , statistics , mathematics
Possible adverse effects of in vitro culture‐associated physical factors were studied in 3‐ and 4‐day‐old rabbit embryos. Laboratory conditions were mimicked by exposure to visible light (320–740 nm, 1600 lx) or decreased temperature (22 ± 1°C). Embryos were exposed for a 24‐hr period followed by either immediate evaluation or an additional 24 hr of standard in vitro culture (darkness, 37°C) and evaluation thereafter. Effects were assayed by cytophotometric measurement of the DNA content in Feulgen‐stained cell nuclei and by cell number. The incidence of DNA aneuploid embryos and DNA aneuploid cell nuclei per embryo, as well as the average nuclear DNA content, was not significantly different between exposed embryos and controls. Both in vitro culture and reduced temperature caused a decrease in cell number. The temperature‐induced cell number decrease was reversible within 24 hr after return to 37°C. These results demonstrate that physical factors associated with in vitro culture do not increase DNA ploidy abnormalities in cultured preimplantation embryos. Mol. Reprod. Dev. 50:30–34, 1998. © 1998 Wiley‐Liss, Inc.