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Mouse spermatogenic cell–specific type 1 hexokinase ( mHk1‐s ) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1‐S protein is localized mainly in the sperm tail
Author(s) -
Mori Chisato,
Nakamura Noriko,
Welch Jeffrey E.,
Gotoh Hideo,
Goulding Eugenia H.,
Fujioka Makio,
Eddy Edward M.
Publication year - 1998
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199804)49:4<374::aid-mrd4>3.0.co;2-k
Subject(s) - biology , somatic cell , alternative splicing , microbiology and biotechnology , spermatogenesis , exon , gene , sperm , cell type , genetics , cell , endocrinology
Unique type 1 hexokinase (HK1) mRNAs are present in mouse spermatogenic cells ( mHk1‐s ). They encode a spermatogenic cell–specific sequence region (SSR) but not the porin‐binding domain (PBD) necessary for HK1 binding to porin on the outer mitochondrial membrane. This study determined the origin of the multiple Hk1‐s transcripts in mouse spermatogenic cells and verified that they are translated in mouse spermatogenic cells. It also showed that a single mHk1 gene encodes the mHk1 transcripts of somatic cells and the mHk1‐sa and mHk1‐sb transcripts of spermatogenic cells, that alternative exons are used during mHk1 gene expression in mouse spermatogenic cells, and that mHK1‐S is translated in mouse spermatogenic cells and is localized mainly with the fibrous sheath in the tail region, not with the mitochondria in the midpiece of mouse sperm. Mol. Reprod. Dev. 49:374–385, 1998. © 1998 Wiley‐Liss, Inc.