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Mouse oocytes suppress cAMP‐induced expression of LH receptor mRNA by granulosa cells in vitro
Author(s) -
Eppig John J.,
Pendola Frank L.,
Wigglesworth Karen
Publication year - 1998
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199803)49:3<327::aid-mrd13>3.0.co;2-t
Subject(s) - biology , luteinizing hormone , follicle stimulating hormone , granulosa cell , oocyte , medicine , endocrinology , follicle stimulating hormone receptor , germinal vesicle , cyclic adenosine monophosphate , antral follicle , messenger rna , ovarian follicle , receptor , gonadotropin , hormone , microbiology and biotechnology , embryo , gene , biochemistry
Mouse oocytes suppress follicle‐stimulating hormone (FSH)–induced luteinizing hormone receptor (LHR) messenger ribonucleic acid (mRNA) expression in cultured granulosa cells. The objective of this study was to assess the mechanism by which oocytes suppress FSH‐induced LHR expression. The effect of cumulus cell–denuded, germinal‐vesicle‐stage oocytes, isolated from antral follicles, on FSH‐induced cyclic adenosine monophosphate (cAMP) production by cultured granulosa cells was determined by radioimmunoassays. In addition, the effect of oocytes on 8Br‐cAMP–induced LHR mRNA steady‐state expression by granulosa cells was assessed by RNase protection assays. Oocytes had no detectable effect on FSH‐induced cAMP production. However, oocytes dramatically suppressed 8Br‐cAMP–induced LHR mRNA steady‐state expression by granulosa cells. It was concluded that the mechanism by which oocytes suppress FSH‐induced steady‐state expression of LHR mRNA is not by inactivating FSH, preventing functional interactions of FSH with its granulosa cell receptors, or by interfering with the signal‐transduction mechanisms required for FSH‐dependent cAMP production. In addition, since oocytes suppressed the 8Br‐cAMP–induced increase in steady‐state expression of mRNA for LHR, oocyte‐derived factors probably suppress expression by acting downstream of FSH‐induced elevation of granulosa cell cAMP. Mol. Reprod. Dev. 49:327–332, 1998. © 1998 Wiley‐Liss, Inc.