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Molecular cloning, genetic mapping, and developmental expression of a bovine transforming growth factor beta (TGF‐β) type I receptor
Author(s) -
Roelen Bernard A.J.,
Van Eijk Michiel J.T.,
Van Rooijen Marga A.,
Bevers Mart M.,
Larson Joshua H.,
Lewin Harris A.,
Mummery Christine L.
Publication year - 1998
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199801)49:1<1::aid-mrd1>3.0.co;2-u
Subject(s) - biology , microbiology and biotechnology , complementary dna , acvr2b , gene , receptor , genetics , tgf beta signaling pathway
A full‐length cDNA encoding the bovine transforming growth factor beta (TGF‐β) receptor type I (bTβR‐I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteine‐rich extracellular domain, and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 96% and 95% identical with its human and mouse homologues, respectively. Genetic mapping assigned the TGFBR1 gene to bovine chromosome 8 at a male genetic distance of 2 centimorgan from D8S28. Assuming conservation of gene order, the linkage data define a breakpoint in mammalian chromosome evolution. Both TGF‐β receptor type I and II mRNAs were found to be expressed in bovine oocytes and preimplantation two‐cell, four‐cell, eight‐cell, morula‐, and blastocyst‐stage embryos, as determined by heminested reverse transcription polymerase chain reaction (RT‐PCR). The mRNA expression patterns of TGF‐β receptor types I, II, and III in a variety of bovine organ tissues were examined by Northern blot hybridization, and highest levels were detected in lung and ovary. Mol. Reprod. Dev. 49:1–9, 1998. © 1998 Wiley‐Liss, Inc.