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Identification of pig primordial germ cells by immunocytochemistry and lectin binding
Author(s) -
Takagi Yuji,
Talbot Neil C.,
Rexroad Caird E.,
Pursel Ver G.
Publication year - 1997
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199704)46:4<567::aid-mrd14>3.0.co;2-t
Subject(s) - biology , immunocytochemistry , lectin , microbiology and biotechnology , identification (biology) , germ , endocrinology , botany
Abstract Monoclonal antibodies anti‐SSEA‐1 and EMA‐1, and the lectins DBA and LTA, bound to the surface of large, round cells randomly distributed in the 26‐day pig genital ridge. Other antibodies, SSEA‐3, SSEA‐4, TRA‐1‐60, and TRA‐1‐81, did not react with any cells in the pig genital ridge. SSEA‐1‐positive cells displayed pseudopods and appeared to migrate from the dorsal mesentery of the hindgut (18‐day) to the primordium of the gonad (day 23) and entered the genital ridge by 26 days. The number of SSEA‐1‐positive cells associated with the dorsal mesentery and genital ridge markedly increased from the 18‐day to the 26‐day pig embryo. It was concluded that the SSEA‐1‐positive cells were primordial germ cells (PGCs). Using these markers and alkaline phosphatase histochemistry, pig PGCs derived from the 26‐day genital ridge showed no proliferation when grown in STO co‐culture in the presence of human LIF, bFGF and SCF. Mol. Reprod. Dev. 46:567–580, 1997. Published 1997 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.