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Effect of estrus‐associated glycoprotein and tissue inhibitor of metalloproteinase‐1 secreted by oviduct cells on in vitro bovine embryo development
Author(s) -
Vansteenbrugge A.,
Van Langendonckt A.,
Massip A.,
Dessy F.
Publication year - 1997
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199704)46:4<527::aid-mrd11>3.0.co;2-t
Subject(s) - blastocyst , oviduct , biology , zygote , embryo , andrology , embryogenesis , glycoprotein , in vitro , embryo culture , immunology , microbiology and biotechnology , endocrinology , biochemistry , medicine
The effect of two glycoproteins (estrus‐associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP‐1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct‐conditioned medium (BOCM) was correlated with the presence of EGP or TIMP‐1. EGP and TIMP‐1 were detected in BOCM, supporting the development of 22% zygotes to the blastocyst stage, as well as in BOCM yielding a low blastocyst rate (3–4% blastocysts). These glycoproteins do not seem to be necessary for bovine embryo development up to the blastocyst stage in our BOCM. In a second experiment, zygotes were cultured in modified synthetic oviduct fluid (mSOF) supplemented with different concentrations (0.5, 5, 50, and 500 μg/ml) of purified bovine EGP. In the third experiment, since purified bovine TIMP‐1 was not available, zygotes were cultured in BOCM depleted of TIMP‐1 by immunoprecipitation treatment. Adding EGP to mSOF, or removing TIMP‐1 from BOCM, did not affect bovine embryo development up to the blastocyst stage, or mean number of cells per blastocyst after 8 days of culture. The results indicate that, under our culture conditions, EGP and TIMP‐1 do not play an important role in sustaining bovine embryo development, and do not influence blastocyst quality, assessed in terms of total number of cells per embryo. Mol. Reprod. Dev. 46:527–534, 1997. © 1997 Wiley‐Liss, Inc.