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Pluripotential rabbit embryonic stem (ES) cells are capable of forming overt coat color chimeras following injection into blastocysts
Author(s) -
Schoonjans Luc,
Albright George M.,
Li JaiLing,
Collen Désiré,
Moreadith Randall W.
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199612)45:4<439::aid-mrd5>3.0.co;2-s
Subject(s) - chimera (genetics) , biology , embryonic stem cell , coat , embryo , microbiology and biotechnology , blastocyst , andrology , induced pluripotent stem cell , stem cell , inner cell mass , in vitro , embryogenesis , immunology , genetics , gene , medicine , paleontology
The isolation of pluripotent embryonic stem (ES) cell lines from preimplantation rabbit embryos and their in vitro properties have been previously described. In the present investigation, these ES cell lines were further characterized and their capacity to contribute to formation of adult, fertile animals upon injection into recipient New Zealand White blastocysts demonstrated. The efficiency of chimera formation was low (5% of live born), but the degree of chimerism, as assessed by coat color contribution from the Dutch belted strain, was high (10–50%). Thus a significant step is taken toward the development of gene‐targeting technology in the rabbit, an animal whose physiology and size lend itself to unique applications in biomedical research. © 1996 Wiley‐Liss, Inc.