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DNA replication initiation by 6‐DMAP treatment in maturing oocytes and dividing embryos from marine invertebrates
Author(s) -
Néant Isabelle,
Dubé François
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199608)44:4<443::aid-mrd3>3.0.co;2-l
Subject(s) - biology , maturation promoting factor , mitosis , meiosis , microbiology and biotechnology , dna synthesis , dna replication , dna , genetics , cell cycle , cell , gene , cyclin dependent kinase 1
6‐dimethylaminopurine (6‐DMAP), a potent protein kinase inhibitor, drives most cells into an interphasic stage. Experiments were undertaken with oocytes from three marine invertebrate species, i.e., Mytilus edulis, Spisula solidissima, and Strongylocentrotus droebachiensis, wherein oocytes were arrested at different phases of meiosis. 6‐DMAP induced a continuous DNA synthesis in meiotic cells, whereas it allowed a single round of DNA replication in treated mitotic cells, regardless of species considered. The effects of 6‐DMAP were accompanied in all cases by rephosphorylation on tyrosine of the p34 cdc2 homolog, the M‐phase promoting factor (MPF) catalytic subunit. The fact that 6‐DMAP overcomes the inhibitory control of replication during meiosis suggests that this process depends upon protein phosphorylation, while DNA synthesis regulation in mitotic cells relies on 6‐DMAP‐insensitive events. © 1996 Wiley‐Liss, Inc.