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Molecular cloning, structural analysis, and expression of carp ZP3 gene
Author(s) -
Chang Y.S.,
Wang S.C.,
Tsao C.C.,
Huang F.L.
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199607)44:3<295::aid-mrd3>3.0.co;2-h
Subject(s) - biology , carp , vitellogenesis , gene , exon , complementary dna , intron , oogenesis , molecular cloning , microbiology and biotechnology , genetics , zona pellucida glycoprotein , coding region , cdna library , cloning (programming) , homology (biology) , oocyte , zona pellucida , embryo , fish <actinopterygii> , embryogenesis , programming language , fishery , computer science
Two types of cDNAs coding for a major component of carp egg membrane were clones from a carp ovarian cDNA library. They encode polypeptides of 422–424 amino acid residues whose sequences are homologous to those of medaka and mammalian ZP3. Similar to the mammalian ZP3 genes, carp ZP3 gene also consists of eight exons and seven introns. Carp ZP3 genes are 2.9 kb in length and present in multiple forms. Carp ZP3 is a glycoprotein of 45 kDa. It was transcribed and translated exclusively in oocytes, in contrast with medaka ZP3, which was synthesized in liver. The transcription of carp ZP3 starts very early in oogenesis, but translation occurs during vitellogenesis, as it is present in vitellogenic but not in previtellogenic oocytes. ZP3 content in oocytes increases as vitellogenesis proceeds. © 1996 Wiley‐Liss, Inc.