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Sperm membrane incorporation into oolemma contributes to the oolemma block to sperm penetration: Evidence based on intracytoplasmic sperm injection experiments in the mouse
Author(s) -
Maleszewski Marek,
Kimura Yasuyuki,
Yanagimachi Ryuzo
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199606)44:2<256::aid-mrd16>3.0.co;2-0
Subject(s) - intracytoplasmic sperm injection , biology , sperm , oocyte , andrology , human fertilization , polyspermy , exocytosis , microbiology and biotechnology , pronucleus , zona pellucida , zygote , penetration (warfare) , anatomy , embryo , embryogenesis , in vitro fertilisation , membrane , genetics , medicine , operations research , engineering
Mouse oocytes were fertilized by intracytoplasmic sperm injection (ICSI) and reinseminated after the removal of zonae pellucidae at pronuclear stage or at the 2‐cell stage. Although these oocytes were activated normally by ICSI, as evidenced by resumption of meiosis and cortical granule exocytosis, they did not develop oolemma block to sperm penetration. They could be penetrated by spermatozoa at pronuclear stage and even at the 2‐cell stage. This supports the notion that incorporation of sperm plasma membrane into oolemma contributes to the changes in oolemma that block sperm penetration. © 1996 Wiley‐Liss, Inc.