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Severe position effects imposed on a 1 kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions
Author(s) -
McKnight Robert A.,
Spencer Mark,
Wall Robert J.,
Hennighausen Lothar
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199606)44:2<179::aid-mrd6>3.0.co;2-k
Subject(s) - scaffold/matrix attachment region , biology , transgene , promoter , gene , heterologous , transcription (linguistics) , microbiology and biotechnology , position effect , transcription factor , gene expression , nuclear matrix , chromatin , genetics , chromatin remodeling , linguistics , philosophy
Matrix attachment regions (MARs) have been shown to participate in the insulation of transcription elements from surrounding chromatin in tissue culture cells and transgenic mice. A whey acidic protein (WAP) transgene containing 1 kb promoter sequence was active in mammary tissue from 1 out of 17 lines of mice, demonstrating that the transcription elements were highly susceptible to position effects. To test whether MARs could insulate this WAP gene promoter and thereby restore transcription, we ligated MARs from the chicken lysozyme gene to the WAP transgene. Seven of the nine lines generated exhibited WAP transgene activity, expression was confined to mammary tissue, and correct regulation was observed in three of the four lines analyzed. This study provides strong additional evidence that the MAR fragments from the chicken lysozyme gene have the capacity to insulate transgenes from severe position effects. © 1996 Wiley‐Liss Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.