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Development‐dependent localization of nuclear antigens in growing mouse oocytes
Author(s) -
Borsuk Ewa,
Vautier Dominique,
Szöllösi Maria S.,
Besombes Didier,
Debey Pascale
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199603)43:3<376::aid-mrd12>3.0.co;2-#
Subject(s) - biology , oocyte , nucleolin , microbiology and biotechnology , ribonucleoprotein , snrnp , cell nucleus , nuclear protein , immunofluorescence , nucleolus , nucleus , rna , genetics , embryo , antibody , transcription factor , gene
We have analyzed the distribution of nuclear and nucleolar proteins during the period of oocyte's growth. Oocytes were isolated mechanically or enzymatically from ovaries of juvenile mice of various ages (from 1 to 28 days after birth). Small nuclear ribonucleo‐proteins (snRNPs), the splicing factor SC‐35, and a protein linked to cell proliferation (p‐120) were detected by indirect immunofluorescence. snRNP distribution is consistent with the prophase state of oocyte's nuclei, while SC‐35 (and p‐120) exhibit a “speckled” distribution throughout the entire period of growth. The number of speckles (or foci) appears maximal around 10 days after birth, i.e., in the period of maximal transcriptional activity, and is sensitive to α‐amanitin treatment. On the other hand, the immunofluorescent distribution of nucleolin and p‐103 (a nucleolar marker of the granular component) is compared to the ultrastructural distribution of the granular component analyzed by electron microscopy on oocytes of the same age. © 1996 Wiley‐Liss, Inc.