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Changes of intracellular free calcium during intracytoplasmic sperm injection
Author(s) -
Leybaert Luc,
Depypere Herman T.,
Dhont Marc,
De Hemptinne Alex
Publication year - 1996
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(199602)43:2<256::aid-mrd15>3.0.co;2-1
Subject(s) - spermatozoon , intracytoplasmic sperm injection , lysis , intracellular , calcium , oocyte , andrology , pipette , sperm , biology , calcium in biology , oocyte activation , human fertilization , microbiology and biotechnology , embryo , medicine , anatomy , chemistry , in vitro fertilisation
The present experiments were undertaken to investigate whether the procedure of intracytoplasmic sperm injection (ICSI) is associated with changes in the intracellular free calcium concentration ([Ca 2+ ] i ). [Ca 2+ ] i was measured, using the calcium‐sensitive dye fura‐2, during and after impalement of mouse oocytes with an ICSI pipette and injection of a small amount of medium alone or of medium containing a normal human spermatozoon. Forty‐five oocytes were injected with medium. Two different responses were observed: 20 of these cells showed a large increase of [Ca 2+ ] i upon impalement; the other 25 cells did not show any change of [Ca 2+ ] i , neither in the acute period nor in a late period 4 hr after impalement. All the cells that responded with an increase of [Ca 2+ ] i subsequently lysed within the first 30 min following impalement, while all the cells with no [Ca 2+ ] i change remained intact. This observation suggests that only traumatic impalement is associated with an increase of [Ca 2+ ] i . Thirty‐one oocytes were successfully, i.e., without subsequent cell lysis, injected with a normal mouse or human spermatozoon. In none of these cells could any acute or late change of [Ca 2+ ] i be observed. The experiments illustrate that successful performance of the ICSI procedure, i.e., ICSI not followed by cell lysis, is not associated with changes of [Ca 2+ ] i in mouse oocytes. This suggests that the ICSI technique, by itself, does not help in activating the oocyte via manipulation‐induced changes of [Ca 2+ ] i . © 1996 Wiley‐Liss, Inc.