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Local application of FFR‐rFVIIa reduces thrombus formation at arterial anastomosis in rats
Author(s) -
Sørensen Hanne B.,
Kristensen Annemarie T.,
Ravn Hanne B.,
Fuglsang Jens,
Hjortdal Vibeke E.
Publication year - 1999
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1999)19:8<369::aid-micr4>3.0.co;2-b
Subject(s) - medicine , arteriotomy , thrombus , antithrombotic , anastomosis , thrombosis , surgery , microsurgery , femoral artery , anesthesia , artery , cardiology
Thrombosis is still a significant problem in microvascular surgery. The aim of this study was to evaluate the antithrombotic effect of topically applied active site‐inhibited recombinant human factor VIIa (FFR‐rFVIIa) in a rat model with microvascular thrombosis. Forty‐five male rats were allocated to one of three groups: local treatment with vehicle only, local treatment with 0.035 mg of FFR‐rFVIIa, or local treatment with 0.35 mg of FFR‐rFVIIa. An arteriotomy was made in the right femoral artery. Ten minutes following topical application, a thrombogenic anastomosis was performed. Using a transilluminator, thrombus formation and anastomotic bleeding episodes were observed and registered for 40 min. Local application of FFR‐rFVIIa resulted in a 85–90% reduction of thrombus formation in both treated groups compared to the control group, but the reduction was only statistically significant in the group treated with 0.035 mg of FFR‐rFVIIa. An increased occurrence and duration of anastomotic bleeding episodes were observed in both FFR‐rFVIIa‐treated groups. © 1999 Wiley‐Liss, Inc. MICROSURGERY 19:369–373 1999