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Intragraft CD45 RO gene expression is an early marker to detect small bowel allograft rejection in rats
Author(s) -
Wang Pengzhi,
Zhu Liwei,
Liu Tong,
Zhang Xijun,
Qiu Yujie
Publication year - 1999
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1999)19:7<348::aid-micr12>3.0.co;2-d
Subject(s) - medicine , histology , tripterygium wilfordii , transplantation , intestinal permeability , gastroenterology , andrology , pathology , alternative medicine
Wistar Furth (WF) intestinal allografts were transplanted into Sprague‐Dawley (SD) rats. Recipients were randomly allocated into the following groups: (1) no treatment; (2) cyclosporine (CsA) 6 mg/kg/day, daily, subcutaneously (s.c.; full‐dose therapy); (3) CsA 3 mg/kg/day, daily, s.c. (half‐dose therapy); and (4) CsA 3 mg/kg/day, daily, s.c. + Tripterygium Wilfordii Hook. WF (TW) 3 mg/kg/day, daily, s.c. WF rats with intestinal autografts were used as controls. CD45RO intragraft expression and its index (CD45RO/CD45), measured by reverse transcription polymerase chain reaction (RT‐PCR), were significantly elevated in untreated and half‐dose CsA‐treated allografts as early as postoperative day (POD) 4, when rejection of intestinal allografts was not detected by routine pathology. Intestinal permeability measured by Tc‐DTPA radioassay was significantly elevated in untreated allografts on POD 6. Histology showed that there was severe rejection in untreated intestinal allografts and mild rejection in allografts treated with a half dose of CsA on POD 6. There was a normal CD45RO expression, permeability, and histology in intestinal allografts treated with either a full dose of CsA or a half dose of CsA + TW. These data indicate that CD45RO intragraft gene expression is an early marker to detect intestinal allograft rejection in rats. © 1999 Wiley‐Liss, Inc. MICROSURGERY 19:348–350 1999