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Significant role of intragraft lymphoid tissues in preventing insulin‐dependent diabetes mellitus recurrence in whole pancreaticoduodenal transplantation
Author(s) -
Tori Masayuki,
Ito Toshinori,
Yumiba Takeyoshi,
Maeda Akira,
Sawai Tsutomu,
Miyasaka Masayuki,
Kiyono Hiroshi,
Matsuda Hikaru,
Nozawa Masumi,
Shirakura Ryota
Publication year - 1999
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1999)19:7<338::aid-micr10>3.0.co;2-k
Subject(s) - medicine , transplantation , islet , allotransplantation , spleen , diabetes mellitus , pancreas , pathology , endocrinology
Graft recurrence of insulin‐dependent diabetes mellitus (IDDM) was examined. Islet transplantation or pancreas‐alone transplantation excluding the duodenum and peripancreatic lymph nodes was compared with whole pancreaticoduodenal transplantation. A Wistar Furth (WF; RT1 u , RT6.2) to major histocompatibility complex (MHC)‐compatible diabetes‐prone (DP; RT1 u , RT6.1 gene carrier)‐biobreeding (BB) rat transplantation model was used. Only DP recipients that had been transplanted with whole pancreaticoduodenal grafts were free from IDDM recurrence (>60 days postgrafting) when treated with anti‐intercellular adhesion moluecule‐1 (ICAM)‐1/leukocyte function‐associated antigen‐1 (LFA‐1) monoclonal antibodies (mAbs). In the spleen cells of the DP rats that had accepted pancreatic grafts (60 days postgrafting), flow cytometric analysis showed that NKR‐P1 + TCRαβ + (NKT) cells had proliferated markedly, with the proportion of 12.8 ± 1.7% in the total splenic T cells, most of which (86.2%) were derived from the donor (RT6.2 + ). By enzyme‐linked immunonosorbent assay (ELISA), serum interferon gamma (IFN‐γ) was not detected (<13 pg/ml) in all rats. However, interleukin‐4 (IL‐4) was detected as 158.8 ± 28.0 pg/ml in the nonrecurrent DP recipients. These data suggested that to prevent IDDM recurrence in the pancreatic graft, the lymphocytes in the pancreaticoduodenal grafts are necessary. Also, the donor‐derived NKT cells might have some immunoregulatory functions with a Th2 deviation. © 1999 Wiley‐Liss, Inc. MICROSURGERY 19:338–343 1999