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Technical experiences with a microsurgical model of lymphatic reconstruction after intestinal transplantation in rats
Author(s) -
Kellersmann Richard,
Grant David,
Zhong Robert
Publication year - 1999
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1999)19:2<108::aid-micr13>3.0.co;2-z
Subject(s) - medicine , lymphatic system , microsurgery , transplantation , surgery , regeneration (biology) , pathology , biology , microbiology and biotechnology
The surgical procedure of intestinal transplantation disrupts the graft's lymphatic drainage. Despite morphological regeneration, the functional impairment of the lymphatic system may contribute to many pathophysiological changes after transplant. We describe the technical details and results of a microsurgical model of lymphatic reconstruction (LR), which immediately reconstitutes lymphatic drainage of the intestinal graft in rats. A total of 42 orthotopic intestinal transplants with LR was performed. The patency of the LR was assessed between postoperative days 2 and 100 using either methylene‐blue staining of the lymphatic vessels or mesenteric lymphoradiography. A total patency rate of 88% was achieved. Genetic background, occurrence of rejection, and immunosuppressive treatment with cyclosporine did not affect the patency rate. Technical aspects and potential applications of this model are discussed. © 1999 Wiley‐Liss, Inc. MICROSURGERY 19:108–112 1999