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Supplement of liver enzyme by intestinal and kidney transplants in congenitally enzyme‐deficient rat
Author(s) -
Kokudo Norihiro,
Takahashi Shigeki,
Sugitani Kazuhiro,
Okazaki Tadaharu,
Nozawa Masumi
Publication year - 1999
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1999)19:2<103::aid-micr12>3.0.co;2-g
Subject(s) - unconjugated hyperbilirubinemia , medicine , transplantation , bilirubin , kidney , endocrinology , gastroenterology , liver transplantation , kidney transplantation
Gunn rats have a congenital deficiency of bilirubin‐uridine diphosphate glucuronyltransferase (B‐UDP‐GT) activity and are unable to glucuronidate bilirubin in the bile, resulting in unconjugated hyperbilirubinemia. Other than the liver, several organs, including small bowel and kidneys, are known to have B‐UDP‐GT activity in normal rats. We performed total‐ or partial‐small‐bowel transplantation as well as kidney transplantation for Gunn rats in congenic combination and compared the effects of these procedures. Serum total bilirubin (TBil) levels significantly decreased from 7.84 ± 0.24 mg/dl to 2.19 ± 0.43 mg/dl 2 weeks after total‐small‐bowel transplantation (n = 12). Correlation of hyperbilirubinemia was roughly proportional to the length of the transplanted small bowel. There were no difference in metabolic correction between jejunal and ileal transplantation. Serum TBil levels significantly decreased from 7.83 ± 0.21 mg/dl to 2.24 ± 0.98 mg/dl 2 weeks after kidney transplantation (n = 5). In conclusion, small‐bowel and kidney transplantation were effective in correcting metabolic abnormality in Gunn rats for the period of 4–6 months. Estimated total B‐UDP‐GT activity supplemented by small‐bowel or kidney transplantation was about 1/5–1/4 of the minimal requirement for the complete normalization of serum total bilirubin levels. © 1999 Wiley‐Liss, Inc. MICROSURGERY 19:103–107 1999