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S‐nitroso‐n‐acetylcysteine protects skeletal muscle against reperfusion injury
Author(s) -
Liu Kang,
Chen LongEn,
Seaber Anthony V.,
Urbaniak James R.
Publication year - 1998
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1998)18:5<299::aid-micr1>3.0.co;2-j
Subject(s) - medicine , microcirculation , skeletal muscle , isometric exercise , cremaster muscle , acetylcysteine , nitric oxide , reperfusion injury , vasodilation , ischemia , stimulation , anesthesia , chemistry , biochemistry , antioxidant
The effects of a nitric oxide (NO) donor on microcirculation and contractile function of reperfused skeletal muscle were studied. Rat cremaster muscles underwent 5 hours of ischemia and 90 minutes of reperfusion and were divided into two groups systemically infused with S‐nitroso‐N‐acetylcysteine (SNAC, 100 nmol/ min) and phosphate‐buffered saline (PBS), respectively. The results showed that the vessels in the SNAC group had more rapid and complete recovery than that in controls. A significant difference was found from 10 to 40 minutes and at 90 minutes in 10–20‐μm arterioles, from 10 to 90 minutes in 20–40‐μm arterioles, and at 10 and 90 minutes in 40–70‐μm arteries. When compared to controls, SNAC‐treated muscles showed larger fluorescein filling areas at 15, 30, 60, and 90 minutes and greater isometric tetanic contractile forces in response to stimulation frequencies of 40, 70, 100, and 120 Hz. The data indicate that supplementation of exogenous NO could effectively improve microcirculation and contractile function of skeletal muscle during early reperfusion. © 1998 Wiley‐Liss, Inc. MICROSURGERY 18:299–305, 1998