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Attenuation of vasospasm and capillary no‐reflow by ischemic preconditioning in skeletal muscle
Author(s) -
Wang Wei Z.,
Anderson Gary,
Maldonado Claudio,
Barker John
Publication year - 1996
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1996)17:6<324::aid-micr7>3.0.co;2-h
Subject(s) - medicine , ischemia , vasospasm , cremaster muscle , anesthesia , perfusion , microcirculation , intravital microscopy , skeletal muscle , arteriole , ischemic preconditioning , cardiology , subarachnoid hemorrhage
Vasospasm and capillary no‐reflow are common complications following replantation and free flap transfer. The purpose of the present study was to clarify whether vasospasm and capillary no‐reflow which are induced by prolonged warm ischemia/reperfusion can be attenuated by ischemic preconditioning in the vascular isolated cremaster muscle model. Male Sprague‐Dawley rats were anesthetized with pentobarbital. Arteriole diameter and capillary perfusion were measured utilizing intravital microscopy. In the control group, the cremasters sustained 4‐hour warm global ischemia followed by 60‐minute reperfusion. In the ischemic preconditioning group, the cremasters were subjected to one cycle of 45‐minute ischemia followed by 15‐minute reperfusion prior to 4‐hour warm global ischemia followed by 60‐minute reperfusion. The results from this experiment showed that ischemic preconditioning significantly attenuated ischemia/reperfusion‐induced vasospasm and capillary no‐reflow which occur early during reperfusion after prolonged warm ischemia in skeletal muscle. The mechanism of this phenomenon remains to be elucidated. MICROSURGERY 17:324–329 1996 © Wiley‐Liss, Inc.