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Microsurgery in a rat lung transplant model: Analysis of benefit
Author(s) -
Yano Osvaldo J.,
Baradarian Robin,
Kim Eugene S.,
Smith Tami J.,
Smith Mark L.,
Smith Craig R.,
Chiu David T.
Publication year - 1996
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/(sici)1098-2752(1996)17:1<25::aid-micr5>3.0.co;2-v
Subject(s) - medicine , animal model , microsurgery , lung , experimental animal , lung transplantation , animal study , transplantation , rat model , hemodynamics , rodent model , cardiology , surgery , veterinary medicine
With the use of microsurgery, we have developed a method of measuring hemodynamic parameters in a rat not possible with previous technology. Three groups of rats were studied: a chemically induced pulmonary hypertensive group (PH); a chemically induced pulmonary hypertensive group treated with single lung transplantation (LT); and an untreated, control group (C). Cardiac output, heart rate, and pulmonary vascular resistance were then calculated in each group from data obtained by 1 mm high fidelity micromanometers and an ultrasonic flow probe. The results show that the data col‐lected from the rodent model are reproducible within each group, and data quality is comparable to large animal models. With this new method, data can be collected in a small animal model at a fraction of the time and cost of large animal studies. Additionally, the complications of graft rejection in large animal studies are eliminated in an isogenic rodent model. © 1996 Wiley‐Liss, Inc.