Premium
Expression analysis of thirty one Y chromosome genes in human prostate cancer
Author(s) -
Lau YunFai Chris,
Zhang Jianqing
Publication year - 2000
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(200004)27:4<308::aid-mc9>3.0.co;2-r
Subject(s) - biology , gene , prostate cancer , genetics , cancer , chromosome , computational biology , cancer research
Rapid advances in positional cloning studies have identified most of the genes on the human Y chromosome, thereby providing resources for studying the expression of its genes in prostate cancer. Using a semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) procedure, we had examined the expression of the Y chromosome genes in a panel of prostate samples diagnosed with benign prostatic hyperplasia (BPH), low and/or high grade carcinoma, and the prostatic cell line, LNCaP, stimulated by androgen treatment. Results from this expression analysis of 31 of the 33 genes, isolated so far from the Y chromosome, revealed three types of expression patterns: i) specific expression in other tissues (e.g., AMELY, BPY1, BPY2, CDY , and RBM ); ii) ubiquitous expression among prostate and control testis samples, similar to those of house‐keeping genes (e.g., ANT3, XE7,ASMTL, IL3RA, SYBL1, TRAMP, MIC2, DBY, RPS4Y , and SMCY ); iii) differential expression in prostate and testis samples. The last group includes X‐Y homologous (e.g., ZFY, PRKY, DFFRY, TB4Y, EIF1AY , and UTY ) and Y‐specific genes (e.g., SRY, TSPY, PRY , and XKRY ). Androgen stimulation of the LNCaP cells resulted in up‐regulation of PGPL, CSFR2A, IL3RA, TSPY , and IL9R and down regulation of SRY, ZFY , and DFFRY . The heterogeneous and differential expression patterns of the Y chromosome genes raise the possibility that some of these genes are either involved in or are affected by the oncogenic processes of the prostate. The up‐ and down‐regulation of several Y chromosome genes by androgen suggest that they may play a role(s) in the hormonally stimulated proliferation of the responsive LNCaP cells. Mol. Carcinog. 27:308–321, 2000. © 2000 Wiley‐Liss, Inc.