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Alteration of Egr‐1 mRNA during multistage carcinogenesis in mouse skin
Author(s) -
Riggs Penny K.,
Rho Okkyung,
DiGiovanni John
Publication year - 2000
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(200004)27:4<247::aid-mc1>3.0.co;2-4
Subject(s) - biology , carcinogenesis , epidermis (zoology) , keratinocyte , tumor promotion , messenger rna , 12 o tetradecanoylphorbol 13 acetate , dermis , microbiology and biotechnology , gene expression , extracellular , cancer research , cell culture , endocrinology , gene , biochemistry , signal transduction , anatomy , genetics , phorbol ester , protein kinase c
Immediate early genes, including fos, jun , and early growth response‐1 ( Egr‐1 ), are induced during cellular response to changes in extracellular environment. These immediate early genes are believed to mediate processes of cell growth and differentiation. In particular, Egr‐1 is induced during mitogenic stimulation of a variety of cell types, including fibroblasts, B cells, and epithelial cells. In the present study, we examined Egr‐1 gene expression during multistage carcinogenesis in mouse skin. After a single topical treatment with the tumor promoter 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA) to SENCAR mouse skin, Egr‐1 mRNA was induced, and maximal induction was observed at 2 h in both epidermis and dermis. Induction of Egr‐1 mRNA by TPA was inhibited by fluocinolone acetonide, a potent inhibitor of tumor promotion by TPA. Egr‐1 mRNA was present in primary keratinocytes derived from adult SENCAR mice. The keratinocyte cultures were maintained in low Ca 2+ medium, and Egr‐1 mRNA levels became significantly elevated after the cultures were switched to high Ca 2+ medium. Additionally, a large proportion of primary papillomas and carcinomas generated from SENCAR mice by standard initiation‐promotion regimens exhibited elevated Egr‐1 mRNA compared with normal epidermis. Taken together, these data suggest a possible role of Egr‐1 during multistage carcinogenesis in mouse skin. Mol. Carcinog. 27:247–251, 2000. © 2000 Wiley‐Liss, Inc.