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Increased expression of mutated Ha‐ ras during premalignant progression in SENCAR mouse skin
Author(s) -
RodriguezPuebla Marcelo L.,
LaCava Margaret,
Bolontrade Marcela F.,
Russell Jamie,
Conti Claudio J.
Publication year - 1999
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199911)26:3<150::aid-mc3>3.0.co;2-p
Subject(s) - biology , carcinogenesis , oncogene , point mutation , allele , microbiology and biotechnology , exon , signal transduction , cancer research , mutation , gene , genetics , cell cycle
The ras proto‐oncogene family products are membrane‐associated, guanine nucleotide–binding proteins that serve as a molecular switch for signal transduction pathways in a diverse array of organisms. In the mouse skin two‐stage carcinogenesis model, a specific point mutation in Ha‐ ras codon 61 is responsible for the initiation event. Here we investigated whether Ha‐ras protein and mRNA expression change during premalignant progression. Also, we assessed the Ha‐ ras mutated allele after these changes. To those ends, we analysed the Ha‐ ras expression profiles in normal and hyperplastic skin, papillomas, and squamous cell carcinomas by western blotting, reverse transcription–polymerase chain reaction, and in situ hybridization. Increased levels of Ha‐ ras expression were observed at specific times during promotion. These changes were followed by an increase in the level of expression of the Ha‐ ras mutated allele. These results suggest that increased expression of Ha‐ ras mutated alleles may have an important role during premalignant progression. Mol. Carcinog. 26:150–156, 1999 . © 1999 Wiley‐Liss, Inc.