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cDNA isolation, expression, and chromosomal localization of the mouse Pcph proto‐oncogene
Author(s) -
Recio Juan A.,
Zambrano Norman,
de la Peña Lorena,
Powers Ciaran,
Siwarski David,
Huppi Konrad,
Notario Vicente
Publication year - 1999
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199910)26:2<130::aid-mc7>3.0.co;2-n
Subject(s) - biology , complementary dna , oncogene , open reading frame , hamster , microbiology and biotechnology , peptide sequence , amino acid , messenger rna , genetics , gene , cell cycle
We report here the isolation and characterization of a cDNA from mouse thymus encoding the murine homolog of the protein product of the Syrian hamster Pcph proto‐oncogene. The single open reading frame identified in the cDNA sequence encoded a protein predicted to have 428 amino acids, which shared 93.7% amino acid identity with the Syrian hamster Pcph within the first 412 residues but had a shorter, highly dissimilar C‐terminus. Northern and western analyses revealed that Pcph mRNA and protein were widely distributed in mouse embryo and adult tissues, with the highest expression in adults detected in kidney and liver. The mouse Pcph proto‐oncogene was mapped by linkage analysis to within 3.3±2.3 cM of Pkch‐rs1 on chromosome 12. These data should prove valuable in designing studies to define the cellular function of the Pcph proto‐oncogene. Mol. Carcinog. 26:130–136, 1999. © 1999 Wiley‐Liss, Inc.