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Search for mutations of the hRAD54 gene in sporadic meningiomas with deletion at 1p32
Author(s) -
Mendiola Marta,
Bello M. Josefa,
Alonso Javier,
Leone Paola E.,
Vaquero Jesus,
Sarasa Jose L.,
Kusak M. Elena,
De Campos Jose M.,
Pestaña Angel,
Rey Juan A.
Publication year - 1999
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199904)24:4<300::aid-mc8>3.0.co;2-g
Subject(s) - biology , gene , coding region , microbiology and biotechnology , genetics , allele , genotype , polymerase chain reaction
The hRAD54 gene is related to a family of genes involved in DNA recombination and repair and encodes a protein with DNA helicase activity. hRAD54 has been mapped to 1p32, a region frequently involved in deletions in a variety of tumor types, including atypical and anaplastic meningiomas. To determine whether alterations of hRAD54 are a common event in meningeal tumors, by means of polymerase chain reaction–single‐stranded conformation analysis we examined 29 tumor samples characterized by 1p deletions for hRAD54 mutations. Although 18 tumors displayed allelic loss at the gene region (1p32) as determined by microsatellite marker analysis, the sole coding‐sequence alteration detected corresponded to a T → C transition, with no amino‐acid change. The genotype distribution was 10.34% TT, 44.8% TC, and 44.8% CC, whereas in the normal controls it was 3.77% TT, 13.2% TC, and 83.01% CC, and most meningiomas with 1p32 deletion retained allele C. Another polymorphism due to a T → C change was evidenced at nt 3008, in the 3′ untranslated region. This change was evidenced in all cases we sequenced. These results appear to exclude the involvement of the hRAD54 gene in the pathogenesis of the nontypical meningiomas, although a detrimental effect of the hRAD54 polymorphisms cannot be ruled out. Mol. Carcinog. 24:300–304, 1999. © 1999 Wiley‐Liss, Inc.