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Oncogenic transformation–dependent expression of a transcription factor NF‐Y subunit
Author(s) -
Gu Zhennan,
KuntzSimon Gaëlle,
Rommelaere Jean,
Cornelis Jan
Publication year - 1999
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199904)24:4<294::aid-mc7>3.0.co;2-q
Subject(s) - biology , transcription factor , protein subunit , transcription (linguistics) , microbiology and biotechnology , genetics , transformation (genetics) , expression (computer science) , gene , computer science , programming language , linguistics , philosophy
As a result of differential splicing, one subunit of the nuclear factor Y (NF‐Y) consists of two major isoforms designated short (NF‐YaS) and long (NF‐YaL). In proliferating normal human fibroblasts, NF‐YaL is by far the more expressed isoform. Surprisingly, NF‐YaS was found by immunoblotting to be as prominent as NF‐YaL in simian virus 40 (SV40)–transformed cell derivatives. As a consequence, two NF‐Y/DNA complexes, one containing the long and the other the short isoform, were formed with extracts from transformed cells and a target promoter element in electrophoretic mobility‐shift assays. Only the complex containing NF‐YaL was detected with extracts from normal fibroblasts. Furthermore, the NF‐Y recognition motif contributed to promoter activation in SV40‐transformed cells but not in normal, cells. Our finding links transcription stimulation in transformed cells to quantitative changes in the expression of an NF‐Ya subunit. Mol. Carcinog. 24:294–299, 1999. © 1999 Wiley‐Liss, Inc.