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Differentially expressed transcripts in x‐ray induced lymphomas identified by dioxygenin‐labeled differential display
Author(s) -
van der Houven van Oordt C. Willemien,
Schouten Theo G.,
van der Eb Alex J.,
Breuer Marco L.
Publication year - 1999
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199901)24:1<29::aid-mc5>3.0.co;2-l
Subject(s) - biology , lymphoma , gene , microbiology and biotechnology , transgene , differential display , genetically modified mouse , gene expression , cancer research , genetics , immunology
Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X‐ray–induced lymphomas derived from Eμ‐ pim ‐1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro‐element that was significantly overexpressed in a subset of lymphomas as compared with non‐lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c‐ myc transcripts. An altered expression pattern of a glutathione S‐transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T‐lymphocyte lipase appeared to be overexpressed specifically in lymphoma‐containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development. Mol. Carcinog. 24:29–35, 1999. © 1999 Wiley‐Liss, Inc.