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Characterization of mutant Ha‐ ras gene expression in transformed murine keratinocyte lines grown under in vitro and in vivo conditions
Author(s) -
Volpe John P.G.,
Conti Claudio J.,
Siaga Thomas J.
Publication year - 1996
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199612)17:4<202::aid-mc3>3.0.co;2-f
Subject(s) - biology , mutant , cell culture , in vivo , microbiology and biotechnology , keratinocyte , in vitro , gene expression , gene , genetics
We investigated the Ha‐ ras and Ki‐ ras gene status, tumorigenicity, pathology, line derivation, and intercellular communication of 7,12‐dimethylbenz[a]anthracene‐initiated papilloma‐, carcinoma‐, and hyperplastic skin‐producing cell lines to further characterize them. Six of nine tumor cell lines grown in vitro expressed both mutant and normal Ha‐ ras proteins, and three lines expressed only normal Ha‐ ras . However, when grown subcutaneously in nude mice, seven of the nine lines expressed both mutant and normal Ha‐ ras , one line expressed normal Ha‐ ras , and one line did not grow subcutaneously. One papilloma line, P2/15, appeared to have an inducible mutant Ha‐ ras gene, as it was expressed only in vivo. These findings suggest that mutant Ha‐ ras genes may be lost in only a minor population of tumor lines during growth in culture. Finally, we found that mutant Ha‐ ras gene expression was strongly correlated with tumorigenicity in nude mice and that intercellular communication was strongly correlated with the derivation of the lines. © 1996 Wiley‐Liss, Inc.