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Specific N‐ ras mutation in bone marrow within 48 H of 7,12‐dimethylbenz[ a ]anthracene treatment in Huggins‐Sugiyama rat leukemogenesis
Author(s) -
Osaka Mitsuhiko,
Matsuo Shinji,
Koh Takashi,
Sugiyama Taketoshi
Publication year - 1996
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199607)16:3<126::aid-mc2>3.0.co;2-f
Subject(s) - biology , 7,12 dimethylbenz[a]anthracene , bone marrow , anthracene , mutation , microbiology and biotechnology , cancer research , genetics , immunology , dmba , gene , carcinogenesis , photochemistry , chemistry
7,12‐Dimethylbenz[ a ]anthracene (DMBA)‐induced leukemias in Long‐Evans rats consistently have an A→T transversion at the second base of codon 61 in the N‐ ras gene. This mutation is also detected in the preleukemic stage. To determine when this specific N‐ ras mutation occurs in the early stages of leukemogenesis, we designed the mutant allele‐specific amplification method, which was sensitive enough to detect one mutant cell among 10 6 normal cells. In the study reported here, N‐ ras mutation was found in bone‐marrow cells 2 d after a single DMBA injection and thereafter throughout the preleukemic stage. These results show that DMBA induces a specific N‐ ras mutation soon after one DMBA injection and that this mutation is probably the first event in DMBA leukemogenesis. © 1996 Wiley‐Liss, Inc.