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Influence of human papillomavirus type 16 gene expression on in vitro differentiation of the human teratocarcinoma cell line 2102Ep
Author(s) -
Stöppler Hubert,
Kirchhoff Tatjana,
Kartenbeck Jürgen,
Gissmann Lutz,
Alonso Angel
Publication year - 1996
Publication title -
molecular carcinogenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.254
H-Index - 97
eISSN - 1098-2744
pISSN - 0899-1987
DOI - 10.1002/(sici)1098-2744(199606)16:2<109::aid-mc7>3.0.co;2-d
Subject(s) - biology , teratocarcinoma , cell culture , cellular differentiation , transfection , microbiology and biotechnology , in vitro , gene , cell type , cell , genetics
Human papillomaviruses (HPVs) are known to infect human keratinocytes and cause alterations in epithelial differentiation. We showed in this study that expression of the HPV‐16 genome was able to interfere with the in vitro differentiation of a human simple‐epithelial cell type, the 2102Ep teratocarcinoma cell line. Stable HPV‐16 genome‐expressing 2102Ep cell lines were generated, and subsequent alterations in differentiation were analyzed in comparison with parental 2102Ep cells. We found that in 2102Ep cells phorbol ester‐induced differentiation led to changes in the expression of SSEA antigens, whereas in HPV‐transfected cell lines only minor changes were observed. © 1996 Wiley‐Liss, Inc.