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Direct effects of 3,4‐methylenedioxymethamphetamine (MDMA) on serotonin or dopamine release and uptake in the caudate putamen, nucleus accumbens, substantia nigra pars reticulata, and the dorsal raphé nucleus slices
Author(s) -
Iravani Mahmoud M.,
Asari Daniel,
Patel Jyoti,
Wieczorek Walter J.,
Kruk Zygmunt L.
Publication year - 2000
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(20000615)36:4<275::aid-syn4>3.0.co;2-#
Subject(s) - nucleus accumbens , dopamine , caudate nucleus , putamen , dorsal raphe nucleus , serotonin , chemistry , substantia nigra , 5 ht4 receptor , neuroscience , nucleus , biology , serotonergic , dopaminergic , biochemistry , receptor
We examined the effects of pressure ejected 3,4‐methylenedioxymethamphetamine (MDMA) from a micropipette on direct chemically stimulated release, and on electrically stimulated serotonin (5‐HT) or dopamine (DA) release in the caudate putamen (CPu), nucleus accumbens (NAc), substantia nigra pars reticulata (SNr), and the dorsal raphé nucleus (DRN) brain slices of rat, using fast cyclic voltammetry (FCV). MDMA is electroactive, oxidising at +1100 mV. When the anodic input waveform was reduced from +1.4 to +1.0 volt, MDMA was not electroactive. Using this waveform, pressure ejection of MDMA did not release 5‐HT or DA in brain slices prepared from any of the nuclei studied. MDMA significantly potentiated electrically stimulated 5‐HT release in the SNr and DA release in CPu. In the DRN or in the NAc, MDMA was without effect on peak electrically stimulated 5‐HT or DA release. The rates of neurotransmitter uptake, expressed as t 1/2 , were in all cases significantly decreased after MDMA. The results indicate that MDMA, unlike (+)amphetamine, is not as a releaser of DA or 5‐HT, it is a potent inhibitor of both DA and 5‐HT uptake. Synapse 36:275–285, 2000. © 2000 Wiley‐Liss, Inc.