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CART peptide analysis by Western blotting
Author(s) -
Kuhar Michael J.,
Yoho Laura L.
Publication year - 1999
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(19990901)33:3<163::aid-syn1>3.0.co;2-t
Subject(s) - cart , blot , peptide , hypothalamus , leptin , biology , pituitary gland , endocrinology , synapse , medicine , chemistry , neuroscience , biochemistry , gene , hormone , mechanical engineering , engineering , obesity
CART peptides have been implicated in leptin‐regulated feeding, reward and reinforcement, neurotropism, and other processes. In this Western blotting study, at least six different CART peptides varying from 4 to 14 kD were found in rat brain, pituitary, gut, and adrenal gland. The peptides may be processed differently in different tissues and one species found in the rat was not found in human hypothalamus. The higher molecular weight species are likely to include preproCART and proCART, while lower molecular weight peptides may be CART 55‐102 and 62‐102, physiologically active fragments. Synapse 33:163–171, 1999. © 1999 Wiley‐Liss, Inc.