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High‐resolution Scatchard analysis shows D 1 receptor binding on pyramidal and nonpyramidal neurons
Author(s) -
Davidoff Stephanie A.,
Benes Francine M.
Publication year - 1998
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199801)28:1<83::aid-syn10>3.0.co;2-z
Subject(s) - receptor , neuroscience , neuropil , dopamine , chemistry , dopamine receptor , biophysics , prefrontal cortex , binding site , medicine , endocrinology , biology , central nervous system , biochemistry , cognition
In order to better understand the mechanism of action of atypical antipsychotic drugs (APDs), it is important to clarify how the dopamine system is integrated within local corticolimbic circuits. Toward this end, a high‐resolution (HR) Scatchard technique has been used to measure the relative density (B max ) and affinity (K d ) of D 1 receptors on large neurons (>100 μm 2 ), on small neurons (<100 μm 2 ), and in neuropil (NPL) of rat medial prefrontal cortex (mPFC) and to determine the laminar distribution of these receptors for each neuronal compartment. Using [ 3 H]SCH23390 as a ligand, all K d and B max values were found to be similar indicating that D 1 receptor activity is not preferentially localized to either large or small neuronal subtypes in mPFC. The density of D 1 receptor binding in all three compartments was found to be almost twice as great in layers V and VI, as compared to superficial layers II and III. These results suggest that the blockade of D 1 receptors associated with some atypical APDs may involve both pyramidal and nonpyramidal neurons in the PFC. Synapse 28:83–90, 1998. © 1998 Wiley‐Liss, Inc.