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In vivo characterization of extracellular GABA release in the caudate nucleus and prefrontal cortex of the rhesus monkey
Author(s) -
Kolachana Bhaskar S.,
Saunders Richard C.,
Weinberger Daniel R.
Publication year - 1997
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199703)25:3<285::aid-syn8>3.0.co;2-7
Subject(s) - microdialysis , nipecotic acid , veratridine , caudate nucleus , extracellular , chemistry , gamma aminobutyric acid , in vivo , neuroscience , prefrontal cortex , biophysics , neurotransmitter , biochemistry , biology , receptor , microbiology and biotechnology , organic chemistry , cognition , sodium channel , sodium
Extracellular gamma amino butyric acid (GABA) levels were measured in the caudate nucleus and the prefrontal cortex of the rhesus monkey brain using in vivo microdialysis under isofluorane gas anesthesia. Evoked GABA release was investigated for voltage sensitivity and calcium (Ca 2+ ) dependency. There was a multifold increase in extracellular GABA levels following local perfusion with: (1) high potassium (50 mM, KCl), (2) veratridine (10 μM), and (3) the GABA releasing agent and uptake blocker, (−) nipecotic acid (1 mM). Release of GABA was significantly reduced when veratridine or (−) nipecotic acid were coinfused in Ca 2+ ‐free cerebrospinal fluid (CSF). Coinfusion of nipecotic acid with TTX (10 μM) also resulted in attenuation of evoked GABA release. These results suggest that GABA levels recovered using in vivo microdialysis, from the caudate nucleus and the prefrontal cortex in the rhesus monkey, derive in significant part from vesicular pools and the exocytotic process is both Ca 2+ ‐dependent and voltage‐sensitive. Synapse 25:285–292, 1997. © 1997 Wiley‐Liss, Inc. This article is a US government work and, as such, is in the public domain in the United States of America.