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Expression of D 1 receptor mRNA in projections from the forebrain to the ventral tegmental area
Author(s) -
Lu XinYun,
Churchill Lynn,
Kalivas Peter W.
Publication year - 1997
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199702)25:2<205::aid-syn11>3.0.co;2-x
Subject(s) - ventral tegmental area , nucleus accumbens , ventral pallidum , forebrain , neuroscience , infralimbic cortex , biology , olfactory tubercle , anatomy , retrograde tracing , dopamine , in situ hybridization , prefrontal cortex , nucleus , microbiology and biotechnology , basal ganglia , central nervous system , messenger rna , dopaminergic , biochemistry , cognition , globus pallidus , gene
In situ hybridization was combined with Fluoro‐Gold retrograde labeling to determine if cells projecting from the forebrain to the ventral tegmental area (VTA) express D 1 receptor mRNA. Cell counts were made in the prefrontal cortex, shell of the nucleus accumbens, and ventral pallidum to estimate the percentage of neurons projecting to the VTA that express D 1 receptor mRNA. Retrogradely labeled cells were observed in the infralimbic and prelimbic regions of the prefrontal cortex, and up to 37% of the retrogradely labeled cells expressed D 1 receptor mRNA. Double‐labeled cells constituted up to 89% of retrogradely labeled neurons in the rostral shell and up to 68% in the caudal shell of the nucleus accumbens. The number of retrogradely labeled cells in the ventral pallidum that were double‐labeled ranged from 13% in the rostral to less than 10% in the caudal portions. These data provide anatomical support for a role of D 1 receptors in the reciprocal innervation between the forebrain and VTA. Synapse 25:205–214, 1997. © 1997 Wiley‐Liss, Inc.