Premium
Real‐time measurement of stimulated 5‐hydroxytryptamine release in rat substantia nigra pars reticulata brain slices
Author(s) -
Iravani Mahmoud M.,
Kruk Zygmunt L.
Publication year - 1997
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199701)25:1<93::aid-syn11>3.0.co;2-#
Subject(s) - substantia nigra , neuroscience , pars reticulata , chemistry , medicine , dopamine , psychology , dopaminergic
Fast cyclic voltammetry at a carbon fibre microelectrode was used to measure 5‐HT signals following electrical or chemical stimulation in rat substantia nigra pars reticulata slices. Chemical stimulation with ( + )‐amphetamine or veratrine gave signals which were indistinguishable from those of exogenous 5‐HT. Electrical stimulation of sufficient duration gave voltammetric signals which were characteristic of 5‐HT. Release of dopamine was not detected following either chemical or electrical stimulation. The 5‐HT signals were attenuated by TTX and enhanced by fluvoxamine. It was not possible to demonstrate regulation of 5‐HT release in the SNr by 5‐HT 1B autoreceptors using CGS 12066A or methiothepin. Signal following electrical stimulation were not enhanced by either benztropine or GBR12909, or modified in the presence of either quinpirole or sulpiride. We conclude that 5‐HT release can be detected voltammetrically in the SNr; 5‐HT release is likely to be from axon terminals, but somatodendritic DA release could not be detected. Synapse 25:93–102, 1997. © 1997 Wiley‐Liss, Inc.