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Differential immunohistochemical labeling of G s , G i1 and 2 , and G o α‐subunits in rat forebrain
Author(s) -
Okuhara Dayne Y.,
Lee John M.,
Beck Sheryl G.,
Muma Nancy A.
Publication year - 1996
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199608)23:4<246::aid-syn2>3.0.co;2-7
Subject(s) - forebrain , immunohistochemistry , neuroscience , differential (mechanical device) , microbiology and biotechnology , protein subunit , chemistry , biology , biophysics , biochemistry , physics , gene , central nervous system , immunology , thermodynamics
The anatomical and morphological distribution of the G proteins G o , G i1 and 2 , and G o α‐subunits in rat forebrain sections was determined using immunohistochemical techniques. Diffuse G o labeling occurred in the neuropil throughout the cortex, superficial layers of the entorhinal cortex, thalamus, several white matter fiber tracts, and hippocampus. G i1 and 2 immunareactivity was also located in the neuropil but produced a more fibrous pattern. Fibrous labeling of G i1 and 2 was observed in the cortex, amygdala, hippocampal subfield CA3, and several white matter fiber tracts. Both G o and G i1 and 2 labeling was present in the pencil fibers within the striatum and lateral geniculate nucleus. G s labeling, in contrast to G o and G i1 and 2 , was generally cytoplasmic. Cytoplasmic G s labeling was observed in the thalamus, habenula, dentate, geniculate nucleus, hypothalamus, and hippocampus. Intense G s labeling was observed in the striatum parenchyma, choroid plexus, and infundibular stem. Based on our results, we conclude that the G proteins G o , G i1 and 2 , and G s are anatomically distributed differently throughout the brain. The diffuse neuropil labeling of G o, fibrous neuropil labeling of G i1 and 2 , and cytoplasmic labeling of G s strongly suggests that the G proteins are also differentially distributed morphologically within a neuron. The differential anatomical and cellular location of G proteins in the CNS may contribute to the coupling specificity between neurotransmitter receptors and G proteins. © 1996 Wiley‐Liss, Inc.