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Chronic stimulation of acetylcholine receptors: Differential effects on Na,K‐ATPase isoforms in a myogenic cell line
Author(s) -
Kragenbrink Renee,
Higham Sandra C.,
Sansom Steven C.,
Pressley Thomas A.
Publication year - 1996
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199607)23:3<219::aid-syn11>3.0.co;2-u
Subject(s) - gene isoform , stimulation , carbachol , acetylcholine receptor , chemistry , acetylcholine , myogenesis , receptor , endocrinology , medicine , hyperpolarization (physics) , microbiology and biotechnology , biology , myocyte , biochemistry , gene , organic chemistry , nuclear magnetic resonance spectroscopy
The myogenic cell line, C 2 C 12 , expresses two isoforms of the Na,K‐ATPase catalytic subunit (α1 and α2) following differentiation. Hyperpolarization in myotubes following long‐term exposure to carbachol (Henning et al. (1993) Br. J. Pharmacol., 111:459–464) suggests that the activity of one or both of the isoforms is increased after chronic stimulation of the acetylcholine receptor. We have examined explicitly the abundance of the two isoforms following such an exposure. Immunoblotting with specific antibodies did not detect any changes in the abundance of the α1 isoform after a 3‐day exposure to carbachol (10 μM). In contrast, there was a 50% increase in the abundance of α2. These results suggest that chronic stimulation of the acetylcholine receptor produced a differential effect on the abundance of the α isoforms. © 1996 Wiley‐Liss, Inc.