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Brain region‐specific and time‐dependent decreases of Gα i2 in amygdala‐kindled rats
Author(s) -
Cutz J.C.,
Li P.P.,
Burnham W.M.,
Warsh J.J.
Publication year - 1996
Publication title -
synapse
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.809
H-Index - 106
eISSN - 1098-2396
pISSN - 0887-4476
DOI - 10.1002/(sici)1098-2396(199603)22:3<226::aid-syn4>3.0.co;2-e
Subject(s) - amygdala , neuroscience , psychology , chemistry
Immunoreactivity levels of G protein α‐subunits, Gα s (45 and 52kDa), Gα i1 , and Gα i2 , were determined in plasmamembranes prepared from ipsilateral amygdala‐pyriform cortex (AM/PC), contralateralAM/PC, dorsal hippocampus (DH), and ventral hippocampus (VH) in amygdala‐kindled rats24 h, 1 month, and 3 months after the last seizure. Relative to sham controls,immunoreactivity levels of Gα i2 were significantly reduced in ipsilateralAM/PC and DH, with maximal decreases observed, respectively, at 24 h (26% form controllevels) and 1 month (18% from control levels) postseizure. No significant alterations in thelevels of Gα i1 or either of the Gα s isoforms wereobserved. The reduction in Gα i2 levels was, however, not accompanied bymeasurable changes in Gα i ‐mediated inhibition of adenylyl cyclase, asestimated by GppNHp modulation of forskolin‐activated adenylyl cyclase activity. The presentfindings add support to the hypothesis that long‐term changes in brain functioning followingkindling may involve altered expression and/or function of components of the transmembranesignalling cascade, including G proteins. © 1996 Wiley‐Liss, Inc.