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Urine as another potential source for template DNA in polymerase chain reaction (PCR)
Author(s) -
Hayakawa Sachiko,
Takenaka Osamu
Publication year - 1999
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/(sici)1098-2345(1999)48:4<299::aid-ajp5>3.0.co;2-g
Subject(s) - polymerase chain reaction , urine , biology , dna , microsatellite , urine sample , microbiology and biotechnology , computational biology , genetics , gene , biochemistry , allele
This technical note examines the potential for preparing template DNA in polymerase chain reactions (PCR) from urine in Japanese macaques ( Macaca fuscata ). Microsatellite band patterns from urine samples showed close agreement with those of blood and fecal samples, and only a few hundred μl of urine yielded a template DNA for PCR. This research will increase the opportunity for scientists to examine the genetic backgrounds of their target animals by using non‐invasive sample collection in the wild. Am. J. Primatol. 48:299–304, 1999. © 1999 Wiley‐Liss, Inc.