Premium
In vitro fertilization and preimplantation embryo development of African green monkeys ( Cercopithecus aethiops )
Author(s) -
Sankai T.,
Cho F.,
Yoshikawa Y.
Publication year - 1997
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/(sici)1098-2345(1997)43:1<43::aid-ajp3>3.0.co;2-y
Subject(s) - polyspermy , biology , andrology , in vitro fertilisation , embryo , human fertilization , in vitro , endocrinology , medicine , anatomy , genetics
Ovaries of five adult female African green monkeys were stimulated by repeated administrations of equine chorionic gonadotrophin (eCG), followed by a single administration of human chorionic gonadotrophin (hCG). Oocytes were collected from enlarged follicles 28 h after hCG administration and incubated in vitro for 288 h. Oocytes that had extruded the first polar body were inseminated with spermatozoa that had been incubated for 4 to 6 h in medium with caffeine and dibutyryl cyclic AMP. Of these oocytes, 66% were fertilized and the incidence of polyspermy was 37%. Eighty‐two percent of the fertilized eggs cleaved, with some developing into expanded blastocysts. Am. J. Primatol. 43:43–50, 1997. © 1997 Wiley‐Liss, Inc.