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ELISA for the measurement of serum and urinary chorionic gonadotropin concentrations in the laboratory macaque
Author(s) -
Munro C. J.,
Laughlin L. S.,
Illera J. C.,
Dieter J.,
Hendrickx A. G.,
Lasley B. L.
Publication year - 1997
Publication title -
american journal of primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.988
H-Index - 81
eISSN - 1098-2345
pISSN - 0275-2565
DOI - 10.1002/(sici)1098-2345(1997)41:4<307::aid-ajp3>3.0.co;2-0
Subject(s) - radioimmunoassay , human chorionic gonadotropin , polyclonal antibodies , endocrinology , medicine , luteinizing hormone , urine , gonadotropin , antiserum , urinary system , monoclonal antibody , biology , chemistry , hormone , antibody , immunology
A rapid, sensitive, enzyme‐linked immunosorbant assay (ELISA) for the measurement of chorionic gonadotropin (CG) in serum and urine samples of laboratory macaques is reported. The ligand (CG) is captured by a readily available, widely used, and well‐characterized monoclonal anti‐body (Mab, 518B7) generated against the β subunit of bovine luteinizing hormone (LH). This Mab, while specific for LH, shows very little species specificity, and has been shown to detect LH and CG by radioimmunoassay (RIA) in both human and non‐human primates. A polyclonal antiserum raised in rabbits against human chorionic gonadotropin (hCG) is conjugated to horseradish peroxidase, and is used as the second anti‐body signal. This anti‐hCG antiserum cross reacts with CG secreted by both the human (hCG) and macaque (mCG). The ELISA utilizes hCG as the standard, and results are based on the relative concentrations of mCG in serum and urine. Total assay time is less than 5 hours. Range of the standard curve is 0.002 to 0.5 ng hCG/well, and the least detectable concentration of hCG is 0.0023 ± 0.0007 ng/well. Pregnancy was detected in early pregnant macaques ( M. fascicularis ) on 9 (N = 1/16), 10 (N = 1/16), 11 (N = 1/16), 12 (N = 6/16), 13 (N = 1/16), 14 (N = 4/16), and 15 (N = 2/16) days following the pre‐ovulatory urinary estrone conjugate peak. The detection of pregnancy by urinary mCG occurred approximately 24 to 72 hours after its detection in serum. Am. J. Primatol. 41:307–322, 1997. © 1997 Wiley‐Liss, Inc.