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Probing the genome for new drugs and targets with DNA arrays
Author(s) -
Lillie Jim
Publication year - 1997
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/(sici)1098-2299(199707/08)41:3/4<160::aid-ddr6>3.0.co;2-j
Subject(s) - computational biology , drug discovery , biology , genome , dna , human genome , gene expression , gene , dna sequencing , gene expression profiling , messenger rna , genetics , bioinformatics
Automated fluorescent sequencers have now generated full and partial sequences of several eukaryotic genomes. How can we best use this information to discover new drugs? One important tool is mRNA expression profiling with DNA arrays. Large‐scale high‐density DNA arrays can be created for hybridization experiments with complex probes to determine in one experiment the level of mRNA expression of each arrayed gene. Genes regulated during a physiological response or disease state can now be quickly identified and become candidates for more intense investigation. Conversely, patterns of expression levels can be used as assays for every step in the drug discovery and development process, from target discovery through clinical evaluation. This review summarizes the opportunity presented by combining EST sequencing data with mRNA expression profiling with DNA arrays to find new ways of discovering drugs. Drug Dev. Res. 41:160–172, 1997. © 1997 Wiley‐Liss, Inc.